The photo was taken by boyfriend and father of their 3 biological children, Brad Pitt, according to the Associated Press.
The mother-of-six was photographed in mid-August in the backyard of their French Chateau.
Jolie appears in a sleeveless top, which has been opened to reveal part of her left breast and a tiny hand, belonging to one of her twins, Knox Leon and Vivienne Marcheline, who were born in July.
All of the couples children— Maddox, Pax, Zahara, Shiloh, Vivienne and Knox — are said to appear in the spread. The magazine will go on sale Oct. 21.
The cover headline promises exclusive "private photos" of the actress taken by Brad Pitt.