Beyonce will perform the National Anthem on Monday afternoon for the second inauguration of U.S. President Barack Obama.
Other performers include James Taylor and Kelly Clarkson.
The ceremony will begin at 11:30 a.m. Eastern Time and Beyonce will close it out at 12:34 p.m. before Obama signs official documents.
Over the weekend, Beyonce uploaded black-and-white photos of her practice session of the "Star-Spangled Banner."
Four years ago, Beyonce also took part in history when she sang Etta James’ "At Last" during the President's and First Lady Michelle Obama’s first dance.
Beyonce told Zap2It that although she is one of the most sought after singers in the world, she too gets nervous when it comes to such momentous performances.
"I can't really think about it until it is over. It is too much! There is no way I would be able to sing," she said. "I know it, but it is the type of thing doesn't matter how many times you sing it. And the biggest fear is making sure you sing it right, and sing it correctly.
The singer added, "I am practicing. I have the tour, the Super Bowl and National Anthem. I am rehearsing right now. I feel like I love this song. Of course it is really emotional and nothing is more emotional than when singing it for a cause, and I know -- the day after tomorrow.
"I don't want to think about it! I love the song because the melody is so beautiful, and you have so many choices. I want to keep it really elegant.”
The 31-year-old beauty also discussed the significance of singing the National Anthem on Martin Luther King Jr. Day.
"Every time I am overworked and in a stressful situation, I always think about moments like this. And it makes everything worth while," she said.
"My father grew up in Alabama and my father was escorted to school with security guards. He was one of the few African-Americans in school. There is so much history that we have overcome. And we are growing so much. It is so beautiful that I can make my family and my family's legacy proud and it is a huge opportunity, for me."
Check out these photos of Beyonce practicing the National Anthem for President Obama’s Inauguration.