The group conducted what it called a virtual teardown of the iPad, since the device is not yet available and component suppliers have not been announced.
Apple's iPad, which resembles a large iPhone and uses the same operating system, will go on sale as early as next month, as the company looks to define a new category of mobile devices.
For the $499 iPad -- the lowest-cost model, which features 16 gigabytes of flash memory -- iSuppli estimated the total materials cost at $219.35, with a $10 manufacturing cost.
The priciest iPad will cost around $335 to produce, while the mid-range model will cost roughly $287, the group said.
The most expensive component in the iPad is the 9.7-inch display and touchscreen, at an estimated cost of $80, according to the estimate.
Analysts expect Apple to ship somewhere between 2 million and 5 million iPad units in the first year.
(Reporting by Gabriel Madway; editing by Andre Grenon)