Designed by Sarah Burton for Alexander McQueen, the intricately decorated dress won over the fashion press and public as soon as Middleton stepped onto the aisle of Westminster Abbey In April.
The bridal gown featured lace applique floral detail and was made of ivory and white satin gazar, with a skirt that resembled an opening flower with white satin gazar arches and pleats. Its train measured just 9ft - modest in comparison with many previous royal brides.
In total, a record 643,000 people are expected to take the tour, during which visitors can see 19 state rooms.
Other than the dress, Middleton's wedding shoes, earrings and a silk replica of her bouquet will also be put on display.
Above the dress is the original veil and Cartier halo tiara worn by the bride, featuring around 900 diamonds and lent to her for the occasion by the queen.
Even the wedding cake is on show in a glass cabinet in the state dining room, complete with ornate sugar flowers and a deep cut where the couple plunged in the knife.