Chelsea take a 1-0 lead from the first-leg at Stamford Bridge last week, yet Barcelona remain favorites to reach the final next month in Munich.
The big surprise in the starting lineups is that Dani Alves has been left out, with Pep Guardiola electing to start with a back three. Isaac Cuenca comes into to provide the width in an advanced role. Cesc Fabregas and Alexis Sanchez return after being left out from El Clasico, while Gerard Pique is also back.
Conversely Chelsea has named the same team that came away from Stamford Bridge last week. Didier Drogba has been named in the lineup, despite concerns over a knee injury.
Barcelona: Valdes, Puyol, Mascherano, Pique, Busquets, Xavi, Iniesta, Cesc, Alexis, Messi, Cuenca.
Chelsea: Cech; Ivanovic, Cahill, Terry, Cole; Meireles, Mikel, Lampard, Ramires, Mata; Drogba.