The “worst heroes ever” will be making their way to the big screen this August when villains of the DC Universe are brought together in “Suicide Squad.” A new trailer dropped during the 2016 MTV Movie Awards Sunday night, featuring clips of Batman (Ben Affleck) and the Joker (Jared Leto) and scenes showing the interesting dynamic within the squad.

The third trailer throws a lot at fans in two minutes and 24 seconds, and there are several moments that viewers will want to keep an eye out for. Here are five moments you may have missed in the all-new “Suicide Squad” trailer:

1. Batman

Ben Affleck’s Batman is featured several times in the new trailer. The Dark Knight is on the roof of the car the Joker drives around in, while also chasing after him in the Batmobile. A woman dressed in blue falls from a bridge in the video, and Batman jumps into the water after her to try to save her. He walks back to the Batmobile with a woman in his arms, but it’s unclear if he’s carrying the person he was trying to save or Harley Quinn (Margot Robbie).

2. More Joker

The Joker has many fans excited to see “Suicide Squad” so they can see the latest onscreen version of the iconic villain. Small glimpses of the Joker were shown in the first two trailers, and he has even more screen time in the latest one. The Joker’s laugh can be heard throughout the video while he is driving around, throwing grenades or sitting at a table threatening someone.

“Are you sweet-talkin’ me?” the Joker asks. “All of that chit-chat is going to get you hurt.”

3. Superman Mention

Batman v Superman: Dawn of Justice” helped launch the new DC Cinematic Universe, and it seems like Batman won’t be the only one with a role in the Suicide Squad. The latest trailer begins with the government talking about a scenario where Superman (Henry Cavill) could rip off the roof of the White House and capture the president. An official asks who could stop him from doing that as members of the Suicide Squad are shown. Could this mention leave the door open for a Superman cameo?

4. El Diablo

El Diablo (Jay Hernandez) got some time to shine in the new trailer. Deadshot (Will Smith) was trying to heat up the firestarter by telling him he “wants to see something.” El Diablo quickly responds by shooting flames out of his hands while the rest of the Suicide Squad watches. After seeing what he can do, Deadshot apologizes and makes sure they’re still cool, well as cool as El Diablo can be.

5. Will The Squad Turn on Each Other?

There are several questions about how teamwork can be when you put together a group of some of the worst criminals. While the trailer showed a brief argument between El Diablo and Deadshot, Katana (Karen Fukuhara) made a move that looked like she was targeting her own squad. In a brief scene, it appears as though Katana is slicing through the head of Killer Croc (Adewale Akinnuoye-Agbaje) with her sword. Looks like fans can expect a bit of infighting with these DC villains.

“Suicide Squad” hits theaters Aug. 5.