Diplomats and journalists were barred again from the court inside Insein prison in Myanmar, a day after the military government opened the trial for the first time since it began on Monday.
The gesture at transparency failed to ease international pressure on the regime to free Nobel Peace laureate Suu Kyi.
Suu Kyi will be jailed up to five years if she is found guilty of breaking the terms of her house arrest.
Critics say the scripted trial is aimed at silencing the charismatic leader of the National League for Democracy (NLD) until after a multi-party election in 2010.
We are happy that the Myanmar authorities let our people see Daw Suu Kyi, but it's not the end, Thai Foreign Minister Kasit Piromya said in Bangkok.
Our main objective is the release of all political prisoners that will lead to national reconciliation, he said.
The recently ill Suu Kyi appeared healthy and confident during the 45-minute hearing on Wednesday attended by 29 diplomats and 10 Burmese journalists. She said she hoped to see them in better days.
Singapore ambassador Robert Chua said she told them national reconciliation was still possible if all parties so wished and it was not too late for something good to come out of this unfortunate incident.
In Washington, Secretary of State Hillary Clinton said the upcoming election would be illegitimate because of the treatment of the charismatic NLD leader.
It is outrageous that they are trying her and that they continue to hold her because of her political popularity, Clinton told a congressional hearing.
Suu Kyi has been detained for more than 13 of the past 19 years, most of them at her home in Yangon, guarded by police, her mail intercepted and visitors restricted.