The ladies of Kappa Kappa Tau better watch their backs because a menacing murderer will try to stick a knife in them during Season 2 of “Scream Queens.”

So when will fans of the Fox comedy watch their favorite sorority girls try to outsmart a killer — again? Well, the network has recently given the sidesplitting series a premiere date. The sophomore season of “Scream Queens” will air Tuesday, Sept. 20, at 9 p.m. EDT, which means you better call dibs on the remote now!

And while audiences should anticipate Chanel (Emma Roberts), Hester (Lea Michelle) and Zayday (Keke Palmer) to run for their lives or die trying in the forthcoming installment, they can also expect fresh new faces to get covered in blood this fall. TVLine revealed that John Stamos, who previously worked with the show’s co-creator Ryan Murphy on “Glee,” has been added to the cast as a series regular.

Stamos had previously starred on the Fox series “Grandfathered,” which was canceled in May after just one season. But despite the show’s cancellation, Stamos’ schedule remains quite full with the production of “Fuller House.” Not only does the actor star in the Netflix original series, but he also produces the “Full House” reboot, which is currently filming its second season.

The actor, who plays Uncle Jesse on “Fuller House,” will swap his iconic catch phrases for a pair of surgical gloves when he makes his debut on “Scream Queens.” Stamos will take on the role of Dr. Brock Holt, who has been described as a brilliant and secretive surgeon at the hospital where Season 2 will take place. During Fox’s Upfront event in May, Jamie Lee Curtis, who stars as Dean Munsch on the dark comedy, revealed that the location of Season 2 will add a creepy factor to the storyline.

“I think it’ll be dark. I think it’s going to be very dark,” Curtis told E! News. “It’s in a hospital, [there’s] a lot of darkness in hospitals — dark hallways, scary rooms, sharp objects, a lot of blood, disinfectants.”

The hospital, run by Munsch, is said to be the facility where the most “fascinating and bizarre medical cases are observed.” 

“[Munsch] will take on the cases no one else will take and the conditions everyone says are incurable,” Brad Falchuk, an executive producer on “Scream Queens,” revealed during the 2016 PaleyFest in March.

“Scream Queens” Season 2 The ladies of Kappa Kappa Tau will try to outrun a new killer in Season 2 of “Scream Queens.” Photo: Fox

“Scream Queens” will return with more bloody good drama when Season 2 premieres Tuesday, Sept. 20, at 9 p.m. EDT on Fox.