Captain America: Civil War
The second trailer for "Captain America: Civil War" features many new glimpses at what fans can expect from the movie when it's released on May 6, 2016. Marvel Entertainment

Sure, the Super Bowl is a big deal, but for many Marvel fans, the release of a new “Captain America: Civil War” trailer is just as big. The latest teaser for the superhero film aired during Sunday's championship game, laying out the sides for the high-stakes battle between former allies.

The trailer is less than a minute long but still packs a punch in setting up one of the most highly anticipated Marvel Cinematic Universe films since the original “Avengers” in 2012. With Captain America (Chris Evans) and Iron Man (Robert Downey Jr.) leading opposing factions, the film promises a break from the typical hero-vs.-villain dichotomy that’s been prevalent in Marvel’s 12-movie franchise so far. To help make sure fans didn’t miss a thing in the dense teaser, below is a rundown of some of the top moments from the 2016 Super Bowl ad.

The Winter Soldier Or Bucky? (0:02)

The previous trailer, as well as the post-credits scene from “Ant-Man,” painted Cap’s long-lost friend, Bucky (Sebastian Stan), as a reformed bad guy in need of the star-spangled hero’s help. But the new trailer finds the Winter Solider suited up in a very familiar setting. In the second of his standalone movies, Captain America learned that his friend had been kept alive like a Frankenstein monster since going MIA in World War II. When he abandoned his programming at the end of the movie, it looked like he was done with all that scientific enhancement. But he appears in the new trailer getting juiced up to do battle against an unknown villain. Perhaps he’s not ready to be his best friend’s do-gooder sidekick just yet.

Scarlet Witch Is Upset (0:08)

According to the film’s official plot synopsis, the split between anti-registration and pro-registration heroes kicks off after the new team of Avengers that Cap leads at the end of “Age of Ultron” is directly responsible for a calamitous incident that results in civilian casualties. This led outlets like CinemaBlend to speculate that the event may be caused by Scarlet Witch (Elizabeth Olsen) and her powers. Although she learned to control them in the “Avengers” sequel, she’s still relatively new at being a superhuman. While it’s a stretch to assume that her covering her mouth in terror is proof of those theories, it’s certainly evidence in support of them. Sadly, the cause of the superhuman destruction probably will not be revealed to those that don’t pay a ticket price to see the film when it drops on May 6.

The Shot (0:10)

Bucky’s inclusion in the movie is curious as he has no real loyalty to anyone except Cap. That’s very clear in the new trailer, which teases a fight between the Winter Soldier and Iron Man. Bucky gets close to the billionaire, but Stark is never caught without his trusty technology to save him. His watch turns out to be a power glove that he uses to grab and absorb a bullet that the Winter Solider was planning to put right through his head. The moment lasts only a second, but the look on Stark’s face when he realizes that the man in front of him is an assassin is enough to win over even the most reluctant Team Iron Man supporter.

Not Rhodey (0:15)

Those that tuned into the first “Captain America: Civil War” trailer already know that something terrible will happen to Iron Man’s best friend, James Rhodes (Don Cheadle). The man who operates the infamous War Machine suit will fight alongside his buddy, but doesn't fare as well in the battle. In the new trailer he lays unconscious with a nose bleed and Iron Man looks pretty sad when he takes off his mask to find his injured, possibly dead, friend. Previously, Cheadle teased that his character will have a pivotal story arc in the film, but stopped short of revealing whether this means Rhodes is making his last appearance.

Ant-Man And The Vision Arrive (0:22)

The teams for both the conflict were previously announced, but they were depicted in full for the first time in the Super Bowl trailer. While Ant-Man (Paul Rudd) and the Vision (Paul Bettany) were announced as members of Team Cap and Team Iron Man respectively, they haven’t been shown in any way until now. One interesting thing about Ant-Man is that he clearly got a suit upgrade since he was last seen in his 2015 debut movie “Ant-Man.” The most prevalent new feature is his slightly more chrome helmet. As for Vision, he doesn’t appear to be sporting any new upgrades. Instead, he's just floating behind Iron Man’s team, ready for the inevitable fight that’s about to ensue.

What was your favorite part of the new “Captain America: Civil War” trailer? Will you be seeing it when it comes out on May 6, 2016? Comment below or tweet your thoughts to @TylerMcCarthy.