Turkish Prime Minister Ahmet Davutoglu, center, prays at a funeral for eight victims of the Feb. 17 car bombing at the Kocatepe Mosque, Feb. 19, 2016, in Ankara. Chris McGrath/Getty Images

Turkish Prime Minister Ahmet Davutoglu on Saturday called on the United States to give unconditional support in the fight against Syrian Kurdish militants, illustrating growing tension between Ankara and Washington over policy in northern Syria.

Davutoglu also said Turkey would tighten security across the country, especially the capital, after a car laden with explosives was detonated near military buses in Ankara on Wednesday, killing 28 people.

Turkey says the Syrian Kurdish YPG, which the United States is backing in the fight against Islamic State group in Syria, was involved in the bombing, working with the outlawed Kurdistan Workers Party (PKK).

Washington, which does not consider the YPG a terrorist organization, has said it is not in a position to confirm or deny Ankara's charge the militia was behind the bombing.

“The only thing we expect from our U.S. ally is to support Turkey with no ifs or buts,” Davutoglu told a news conference following a five-hour security meeting with members of his cabinet and other officials.

“If 28 Turkish lives have been claimed through a terrorist attack we can only expect them to say any threat against Turkey is a threat against them.”

The disagreement over the YPG risks driving a wedge between the NATO allies at a critical point in Syria's civil war as the United States pursues intensive talks with Syrian ally Russia to bring about a “cessation of hostilities.”

The Kurdistan Freedom Hawks (TAK), a group that once had links to the PKK, Friday claimed responsibility for the bombing. However, Davutoglu said that did not rule out the responsibility of the YPG, calling the TAK a “proxy” that claimed the bombing to shield the international reputation of the Syrian Kurdish fighters.

President Barack Obama on Friday spoke to Turkish President Tayyip Erdoğan in an 80-minute telephone call, sharing his concerns over the Syrian conflict and promising his support.

On Friday, a State Department spokesman told reporters Washington would continue to support organizations in Syria that it could count on in the fight against the Islamic State group — an apparent reference to the YPG.